[Bio-Linux] Live Bio-Linux not persistent

Thu Jun 12 12:12:06 EDT 2014

Dear All,

   Just for your information, their is an alternative way to create 
Bio-Linux live USB drives. Universal usb installer allows you to create 
live usb for any Linux os in minutes.

For Bio-Linux just select the option for Ubuntu 64 bit. I have several 
bio-Linux  live USB made with tool and never had any issues with it.

You can access the tool for free here:
http://www.pendrivelinux.com/universal-usb-installer-easy-as-1-2-3/

Regards,
James

On 6/12/2014 4:12 AM, Cox, Michael J wrote:
> Dear All,
>
> I think I fixed this, when I checked /boot/grub/grub.cfg there was no 
> persistent flag. Adding this to the first menu entry line:
>
> linux /casper/vmlinuz.efi file=/cdrom/preseed/ubuntu.seed boot=casper 
> *persistent* quite splash ---
>
> now renders the sticks persistent.  In addition I can now see the data 
> and tutorials that Tim loaded for me in /home.
>
> It does take a little longer to load and is slow initially, so I have 
> some concerns that I have done something less than ideal.  Comments 
> appreciated.
>
> Thanks
>
> Mike
>
>
> On 12 Jun 2014, at 12:00, <bio-linux-request at nebclists.nerc.ac.uk 
> <mailto:bio-linux-request at nebclists.nerc.ac.uk>> 
> <bio-linux-request at nebclists.nerc.ac.uk 
> <mailto:bio-linux-request at nebclists.nerc.ac.uk>> wrote:
>
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>>   1. Live Bio-Linux not persistent (Cox, Michael J)
>>
>>
>> ----------------------------------------------------------------------
>>
>> Message: 1
>> Date: Thu, 12 Jun 2014 09:13:37 +0000
>> From: "Cox, Michael J" <michael.cox1 at imperial.ac.uk>
>> To: "bio-linux at nebclists.nerc.ac.uk" <bio-linux at nebclists.nerc.ac.uk>
>> Subject: [Bio-Linux] Live Bio-Linux not persistent
>> Message-ID: <B6B32EFF-5AD6-436B-B760-975F4CCA4864 at imperial.ac.uk>
>> Content-Type: text/plain; charset="windows-1252"
>>
>> Hi All,
>>
>> we?re using live USBs for a course next week that Tim kindly cloned 
>> after installing a couple of R packages and a test dataset.  He?s now 
>> on his hols, so I thought I?d check to see whether the rest of the 
>> list could help me with an issue that I?m having.
>>
>> On boot, I couldn?t find the data that Tim added.  I?ve checked by 
>> creating a file and the sticks aren?t persistent and files are 
>> removed when the USB stick is.  I did find a reference that suggested 
>> creating a new user and that user account would be persistent, but no 
>> joy, new user and file removed on reboot.
>>
>> The additional R packages that Tim added to the distribution (dev 
>> version of PhyloSeq) are present and correct, so that at least has 
>> stayed.
>>
>> I have checked in:
>>
>> /syslinux/txt.cfg
>>
>> and this seems correct - the persistent flag is set on the Try 
>> Bio-Linux 7 option:
>>
>> default live
>> label live
>>  menu label ^Try Bio-Linux 7 without installing
>>  kernel /casper/vmlinuz.efi
>>  append noprompt cdrom-detect/try-usb=true persistent 
>> file=/cdrom/preseed/ubuntu.seed boot=casper initrd=/casper/initrd.lz 
>> quiet splash  locale=en_GB.UTF-8 --
>> label live-install
>>  menu label ^Install Bio-Linux 7
>>  kernel /casper/vmlinuz.efi
>>  append noprompt cdrom-detect/try-usb=true persistent 
>> file=/cdrom/preseed/ubuntu.seed boot=casper only-ubiquity 
>> initrd=/casper/initrd.lz quiet splash --
>> label check
>>  menu label ^Check stick for defects
>>  kernel /casper/vmlinuz.efi
>>  append  boot=casper noprompt integrity-check 
>> initrd=/casper/initrd.lz quiet splash --
>> label memtest
>>  menu label Test ^memory
>>  kernel /install/mt86plus
>> label hd
>>  menu label ^Boot from first hard disk
>>  localboot 0x80
>>
>> This post 
>> http://ubuntuforums.org/showthread.php?t=2124124&p=12551088#post12551088 
>> suggests editing syslinux.cfg (rather than txt.cfg) to make a Ubuntu 
>> stick persistent, which I?ve tried, but it booted up exactly as 
>> before and again was stubbornly resistant to retaining files.
>>
>> Can anyone suggest ways to get this working?  Given the fact the R 
>> packages have remained, some aspect of this seems to be functioning? 
>>  I could have the attendees download the data and course material, 
>> but would much rather they left with a copy of Bio-Linux and all 
>> their work and notes in one elegant package.
>>
>> Thanks very much for your help
>>
>> Mike
>>
>>
>> Mike Cox
>>
>> Research Associate
>> Molecular Genetics & Genomics
>>
>> Genomic Medicine
>> National Heart and Lung Institute
>> Guy Scadding Building
>> Royal Brompton Campus
>> London SW3 6LY
>>
>> 0207 594 7974
>>
>> http://www1.imperial.ac.uk/medicine/people/michael.cox1/
>>
>> * New NHLI short course "Principles of Genomic Medicine" *
>> http://www1.imperial.ac.uk/nhli/PGM
>>
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