[Bio-Linux] Pregap4/Gap4 problems

[Jose Andres]

Hi all,

I am using pregap4/gap4 to assemble a BAC and I have problems adding  
sequences to to an existing database....

When running pregap4 (Phrap assembly) I get the following error  
"database busy" (even if no one is using it!) I have deleted  
the .BUSY file and run pregap again. This time I do not get any  
errors but the sequences that passed are not added (the new sequences  
are not into the pregap.assembly directory even if the pregap4 output  
window reads "assembled data written to directory ../pregap.assembly"  
and no error messages are generated). Any idea of what is going on?  
Any suggestions?

  -- I am at the beginning of the project and most of the sequences I  
have are singlets (can that be part of the problem?) ---


Thanks in advance,

/Jose